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NMR
Spectroscopy with VNMR Albin Otter January 2007 (HTML version 07-01) for VNMRJ click here |
1. Objectives
2. Introduction
3. Overview of
UNIX-based spectrometers and computers
4. NMR
system overview
4.1 Probes and other hardware
4.2 Sensitivity and spectral dispersion
5. Sample preparation and pre-acquisition activities
5.1 Sample preparation
5.2 Sample volume and sample concentration
5.3 Spectral references
5.4 Access to spectrometers (reservations etc.)
5.5 Logging into the system
5.6 The VNMR interface
5.7 Tuning, locking and shimming5.7.1 Inserting the sample
5.7.2 Locking
5.7.3 Tuning
5.7.4 Shimming
6. Data Acquisition using 1:AO menu system
6.1 Example: recording of a 1D proton and GCOSY in D2O on i300
6.1.1 1D spectrum (acquisition in experiment #1)
6.1.2 1D spectrum (partial processing in experiment #1)
6.1.3 2D GCOSY (acquisition in experiment #2)
6.1.4 1D spectrum (full processing in experiment #1)
6.1.5 2D GCOSY (full processing in experiment #2)
7. Post-acquisition activities
7.1 Saving data
7.2 Exit VNMR and log out
9. Overview of the most important VNMR parameters
9.1 Data acquisition 1D (i300, i400, s400, m400, ibd5, u500, i600)
10. Appendix
10.1 FID
10.2 digital resolution
10.3 Sep-Pak filtration for carbohydrates
| Figure 3.1: | Varian spectrometers and UNIX data stations |
| Figure 4.1: | The main components of an NMR spectrometer |
| Figure 4.2: | Three-button mouse |
| Figure 5.1a: | Pasteur pipette with cotton wool |
| Figure 5.1b: | Filtration through cotton wool |
| Figure 5.1c: | NMR request sheet depth gauge |
| Figure 5.2: | NMR tube and sample volume |
| Figure 5.3: | SUN Solaris log in screen |
| Figure 5.4: | The VNMR interface |
| Figure 5.5: | Acquisition Window (initial view) |
| Figure 5.6: | Acquisition Window in Lock mode (not locked) |
| Figure 5.7: | Inserting sample |
| Figure 5.8: | Loading shims |
| Figure 5.9: | Acquisition Window in Lock mode (locked) |
| Figure 5.10: | Acquisition Window in Shim mode |
| Figure 6.1: | AO Acquisition menu buttons |
| Figure 6.2: | Partial view of AO 1D Processing menu buttons |
| Figure 6.3: | AO 1D Processing menu buttons |
| Figure 6.4: | AO Processing menu buttons (integral) |
| Figure 6.5: | AO 2D Processing menu buttons |
| Figure 7.1: | AO Data saving and retrieving menu buttons |
| Figure 7.2: | VNMR file format |
| Figure 7.3: | Suggested file name structure |