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NMR Spectroscopy with VNMR
(and locally developed additions to VNMR)
VNMR version 6.1C

Albin Otter
Department of Chemistry
University of Alberta
Edmonton, Alberta
Canada T6G 2G2

January 2007

(HTML version 07-01)

for VNMRJ click here

Table of Contents

1. Objectives
Overview of UNIX-based spectrometers and computers
NMR system overview

4.1 Probes and other hardware
Sensitivity and spectral dispersion

5. Sample preparation and pre-acquisition activities

5.1 Sample preparation
Sample volume and sample concentration
5.3 Spectral references
5.4 Access to spectrometers (reservations etc.)
Logging into the system
The VNMR interface
Tuning, locking and shimming

5.7.1 Inserting the sample
5.7.2 Locking
5.7.3 Tuning

6. Data Acquisition using 1:AO menu system

6.1 Example: recording of a 1D proton and GCOSY in D2O on i300

6.1.1  1D spectrum (acquisition in experiment #1)
6.1.2  1D spectrum (partial processing in experiment #1)
6.1.3  2D GCOSY (acquisition in experiment #2)
6.1.4  1D spectrum (full processing in experiment #1)
6.1.5  2D GCOSY (full processing in experiment #2)

7. Post-acquisition activities

7.1 Saving data
7.2 Exit VNMR and log out

8. Unsupported solvents

8.1 1D spectra
8.2 2D spectra (example: GCOSY)

9. Overview of the most important VNMR parameters

9.1 Data acquisition 1D (i300, i400, s400, m400, ibd5, u500, i600)

10. Appendix

10.1 FID
10.2 digital resolution
10.3 Sep-Pak filtration for carbohydrates

Table of Figures

Figure 3.1: Varian spectrometers and UNIX data stations
Figure 4.1 The main components of an NMR spectrometer
Figure 4.2: Three-button mouse
Figure 5.1a: Pasteur pipette with cotton wool
Figure 5.1b: Filtration through cotton wool
Figure 5.1c: NMR request sheet depth gauge
Figure 5.2: NMR tube and sample volume
Figure 5.3 SUN Solaris log in screen
Figure 5.4: The VNMR interface
Figure 5.5 Acquisition Window (initial view)
Figure 5.6 Acquisition Window in Lock mode (not locked)
Figure 5.7: Inserting sample
Figure 5.8: Loading shims
Figure 5.9: Acquisition Window in Lock mode (locked)
Figure 5.10: Acquisition Window in Shim mode
Figure 6.1: AO Acquisition menu buttons
Figure 6.2: Partial view of AO 1D Processing menu buttons
Figure 6.3: AO 1D Processing menu buttons
Figure 6.4: AO Processing menu buttons (integral)
Figure 6.5: AO 2D Processing menu buttons
Figure 7.1: AO Data saving and retrieving menu buttons
Figure 7.2: VNMR file format
Figure 7.3: Suggested file name structure

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