Chemical shifts are temperature-dependent. Consequently, if a sample is measured first on i300 and then for some more experiments on i600 or u500, the temperature should ideally be the same, otherwise the shifts will be different. Although the deviations might be small, it can cause unnecessary and very unpleasant confusion. By using the EZ NMR buttons, 27.0°C is automatically achieved on all spectrometers. There might be a significant deviation between the reading on the remote status display and the actual temperature in the probe.

Shims are temperature-dependent. All shims available in the general shim library are for 27°C. The more the temperature is different form 27°C, the less the shims will work.

Quite a number of 1D and 2D experiments available use solvent suppression by presaturation of the solvent peak. The decoupler (or transmitter) can only be effective if the solvent peak is really where the irradiation frequency is set to. Example: the shift of the HDO resonance (4.75 ppm at 27.0°C) changes by about 0.01 ppm per °C. Because the decoupler is set to irradiate at exactly 4.75 ppm, the effectiveness of the presaturation decreases substantially with every degree the temperature is off, i.e. without manually readjusting the irradiation point the presaturation will only be optimum at 27.0°C. This is less of a concern for solvents such as CDCl3, CD2Cl2 and C6D6. In those cases the solvent does not typically require any special treatment.