1. EZ NMR to Simplify NMR |
Documentation for usage of the m400 can also be found at: http://nmr.chem.ualberta.ca. |
2. Introduction |
This document deals exclusively with high-resolution (i.e. liquid not solid state) Linux-based Agilent NMR spectrometers which are all linked through the local Ethernet network. The main objective of this manuscript is to provide information on how to run these spectrometers in a safe and efficient way by using specific locally developed menu panels referred to as “EZ NMR”. With the help of these panels, 1D and 2D data can be acquired, processed and plotted without explicitly entering commands. The explanation of general VNMRJ commands is kept to a minimum as there are excellent manuals located on each spectrometer and data station (on-line pdf manuals). Spectrometer host computers and data stations are equipped with the following manuals:
which are instructive for those wishing to learn more about general VNMRJ commands. Except for the field strength, associated spectral dispersion and sensitivity, the operation of all instruments and data stations is essentially identical as they are all equipped with similar hardware, use the same version of the Linux operating system and use similar versions of VNMRJ software:
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2.1 The NMR Staff and SpectrometersThe Department of Chemistry has 8 high-resolution NMR spectrometers and a small staff of three personnel dedicated to the care and maintenance of these instruments as well as aiding users with anything liquid NMR related. The NMR staff are also available to measure samples for users that require special techniques, long experimental times (dilute samples), require immediate attention (unstable compounds), training new users, plus many other items. |
2.1.1 The NMR StaffThe NMR staff can be found in on the third floor of the East wing, room E3-17A and room WB-13 in the basement of the West wing within the Department of Chemistry. 3rd Floor East Wing: Basement West Wing: |
For concerns with the instruments or data stations please notify the NMR staff immediately in person, by phone, and/or by email. |
2.1.2 The SpectrometersThe rest of the NMR instruments are located throughout the Department and can be found: Sub-Basement:
Basement:
First Floor:
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2.2 Data Stations and Remote Access PointsAAll general access data stations and remote access points behave the same as the spectrometers.Data stations support: archiving data to CD, DVD, USB devices, and remote login via ssh within the Department of Chemistry. Please see the Facility Supervisor for permission to access a data station remotely. Remote access points DO NOT support: archiving data to CD, DVD, USB devices, or remote login via ssh. These systems are for processing and printing data ONLY. Seven general access data stations are available for data processing and archiving data:
One remote access point is available for data processing and printing data only:
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3. NMR System Overview |
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Each spectrometer consists of three main components:
Aside from inserting and removing your sample the entire system is controlled nearly 100% from the spectrometer host computer. |
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3.1 Consoles, probes and other hardware |
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3.2 Sensitivity and spectral dispersion |
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H1 sensitivity and resulting experimental times or sample concentration, respectively, to reach the same signal-to-noise is compared below:
Spectral dispersion is proportional to the field strength hence a 600 is twice as good as a 300. The probability for spectral overlap in a two-dimensional experiment decreases significantly with increasing field strength: 300: 1.0 400: 0.56 500: 0.36 600: 0.25 700: 0.18 |
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