|High Resolution NMR Spectroscopy of Biomolecules
Aside from duties to manage the NMR facility consisting of two technicians, 8 high-resolution spectrometers spread out over 5 different laboratories, professional interests include NMR techniques in solution, especially two- and higher dimensional techniques and their selective equivalents of lower dimensionality. The conformational analysis of biomolecules such as oligosaccharides, glycopeptides, carbohydrate/protein complexes and peptides in solution by NOESY, (T)ROESY and transferred NOESY techniques is a major part of the work.
Equally important is general support to NMR users in the Department, together with training of new students and post docs in basic and advanced NMR techniques. An extensive system of macros, parameter sets and menu buttons (called EZ NMR) to simplify the use of the eight Varian spectrometers in the Department is constantly being updated and extended. Additions and changes to this system are made available via a local newsletter called NMR News and the NMR web site.
The continuous effort to modernize and simplify our large NMR operation and thereby bring it to a level whereby students and post-docs can use it safely and without expert help, resulted in a Faculty of Science Service Award to AO in 2005. The contribution to this achievement by the NMR staff is gratefully acknowledged.
Selected Publications (complete publication list):
|M. Nitz, C.-C. Ling, A. Otter, J. Cutler and D.R. Bundle, The Unique Solution Structure and Immunochemistry of the Candida albicans b-1,2-mannopyranan Cell Wall Antigens, J. Biol. Chem. 277, 3440-3446 (2002).|
|S.A. Wacowich-Sgarbi, C.C. Ling, A. Otter and D.R. Bundle, A Tethered Disaccharide Trapped as Its anti Conformer Calibrates the Karplus Relationship for 3JCH Coupling Constants, J. Am. Chem. Soc. 123, 4362-4363 (2001).|
|X. Qian, K. Sujino, A. Otter, M. M. Palcic and O. Hindsgaul, "Chemoenzymatic Synthesis of a(1- 3)-Gal(NAc)-Terminating Glycosides of Complex Tertiary Sugar Alcohols", J. Amer. Chem. Soc. 121, 12063-12072 (1999).|
|A. Otter, R.U. Lemieux, R.G. Ball, A.P. Venot, O. Hindsgaul and D.R. Bundle, "Crystal State and Solution Conformation of the Blood Group B Trisaccharide a-L-Fucp-(1-2)-[a-D-Galp]-(1-3)] b-D-Galp-OCH3", Eur. J. Biochem. 259, 295-303 (1999).|
|D.R. Bundle, R. Alibes, S. Nilar, A. Otter, M. Warwas and P. Zang, "Thermodynamic and Conformational Implications of Glycosidic Rotamers Pre-Organized for Binding", J. Amer. Chem. Soc. 120, 5317-5318 (1998).|
|A. Otter and D.R. Bundle, "Long range 4J and 5J including inter-glycosidic correlations in gradient-enhanced homonuclear COSY experiments of oligosaccharides", J. Magn. Reson. Ser. B 109, 194-201 (1995).|
|A. Otter, D.R. Bundle and O. Hindsgaul, "Gradient-enhanced homonuclear 2D NMR techniques applied to oligosaccharides containing manno-hexoses provide improved correlations for protons coupled by small 3J", Carbohydr. Res. 275, 381-389 (1995).|
|R.A. Field, A. Otter, W. Fu and O. Hindsgaul, "Synthesis and 1H NMR characterization of the six isomeric mono-O-sulfates of LacNAc-MCO", Carbohydr. Res. 276, 347-363 (1995).|
|A. Otter, P.G. Scott and G. Kotovych, "Conformational analysis of the type I and type II collagen a-1 chain N-telopeptides by 1H NMR spectroscopy and restrained molecular mechanics calculations", Biopolymers 33, 1443 (1993).|
|A. Otter, P.G. Scott and G. Kotovych, "Type I collagen a-1 chain C-telopeptide: solution structure determined by 600 MHz proton NMR spectroscopy and implications for its role in collagen fibrillogenesis", Biochemistry 27, 3560 (1988).|
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